Knockdown efficiency and individual repeats of cell growth assay in cells transfected with siRNA against PTEN ceRNAs.
This is the same experiment as Figure 5. (A) Independent biological repeats of cell growth assay. Dot plot with means reported as crossbars and error bars represent SD. (B) Independent biological repeats of RT-qPCR analysis. Expression of each transcript after transfection of its respective siRNA relative to negative control transfection (siNC) is presented. Transcripts listed on y-axis. Means reported and error bars represent SD from three technical replicates. One-sample t-tests of transcript expression data after transfection of respective siRNA to a constant of 1 (relative value of siNC). DU145 cells: VAPA: t(4) = 58.6, uncorrected p=5.09×10−7, Bonferroni corrected p=1.53×10−6; CNOT6L: t(4) = 36.3, uncorrected p=3.42×10−6, Bonferroni corrected p=1.03×10−5; PTEN: t(4) = 9.05, uncorrected p=8.27×10−4, Bonferroni corrected p=0.0025. Wild-type HCT116 cells: VAPA: t(3) = 18.6, uncorrected p=3.40×10−4, Bonferroni corrected p=0.0010; CNOT6L: t(3) = 108, uncorrected p=1.73×10−6, Bonferroni corrected p=5.19×10−6; PTEN: t(3) = 28.1, uncorrected p=9.90×10−5, Bonferroni corrected p=2.97×10−4. DicerEx5 HCT116 cells: VAPA: t(3) = 14.8, uncorrected p=6.73×10−4, Bonferroni corrected p=0.0020; CNOT6L: t(3) = 27.9, uncorrected p=1.02×10−4, Bonferroni corrected p=3.05×10−4; PTEN: t(3) = 178, uncorrected p=3.89×10−7, Bonferroni corrected p=1.17×10−6. Additional details for this experiment can be found at https://osf.io/5c7sb/.