This is the same experiment as Figure 1. (A) Independent biological repeats of Western blot assay. PTEN/HSP90 protein expression is presented for each condition relative to the siNC condition. (B) Independent biological repeats of RT-qPCR analysis. Expression of each transcript after transfection of its respective siRNA relative to negative control transfection (siNC) is presented. Transcripts listed on y-axis. Means reported and error bars represent SD from three technical replicates. One-sample t-tests of transcript expression data after transfection of respective siRNA to a constant of 1 (relative value of siNC). Wild-type HCT116 cells: SERINC1: t(2) = 8.41, uncorrected p=0.014, Bonferroni corrected p=0.055; VAPA: t(2) = 120, uncorrected p=6.98×10−5, Bonferroni corrected p=2.79×10−4; CNOT6L: t(2) = 10.4, uncorrected p=0.0091, Bonferroni corrected p=0.036; PTEN: t(2) = 30.6, uncorrected p=0.0011, Bonferroni corrected p=0.0043. DicerEx5 HCT116 cells: SERINC1: t(2) = 28.5, uncorrected p=0.0012, Bonferroni corrected p=0.0049; VAPA: t(2) = 48.3, uncorrected p=4.28×10−4, Bonferroni corrected p=0.0017; CNOT6L: t(2) = 3.23, uncorrected p=0.084, Bonferroni corrected p=0.336; PTEN: t(2) = 242, uncorrected p=1.71×10−5, Bonferroni corrected p=6.83×10−5. Additional details for this experiment can be found at https://osf.io/drcbw/.