Preparation of Acyclovir-Loaded Lecithin-Chitosan Nanoparticles
ALN was prepared by the method reported by Sedef and group with little modification (15). An appropriate amount of lecithin and chitosan was weighed, as shown in Table II. Chitosan was dissolved in 15 mL of 0.1% v/v acetic acid followed by heating to 60°C. Lecithin prepared was dissolved in an organic solvent consisting of 2.5 mL methanol, 2.5 mL chloroform, and 2 mL PG. An amount 15 mg of ACV was added after full solubilisation of lecithin, followed by sonication to solubilise all acyclovir. The lecithin/ACV solution was then added dropwise into chitosan solution under vigorous magnetic stirring. Finally, the nano-suspension was probe sonicated for 4 min.