Particle size and PDI obtained from all 17 formulations were recorded in Table IV as observed values and illustrated in Fig. 2 c. The particle size ranged from 155.31 ± 6.87 to 264.82 ± 4.33 nm, with PDI ranged from 0.179 ± 0.03 to 0.429 ± 0.12. In overall, the results were accepted as it is capable of exhibiting enhanced permeability and retention (EPR) effect. Equation (11) describes the effect of three independent factors on particle size, with high r2 value of 0.9162 to prove that the equation is reliable.11 Y2=162.83+16.23X1+18.39X2+16.72X3+14.47X1X2–14.07X1X3–15.65X2X3+17.39X12+26.7X22+47.32X32