Apparent permeability of the formulation as a function of the mucosal surface area was calculated by Eq. (6). In contrast, Eqs. (7) and (8) were used to calculate apparent permeability coefficient and steady-state flux, and Jss of acyclovir in formulation, respectively.6 Apparentpermeabilitymgcm−2=Mmedium/MucosalSurfaceAreacm27 Pappcms−1=dQ/dt1/AC0