The application was done such that Day zero was treated with 50% of the bioremediation cocktail after which soil sample was taken for TPH and THC measurement, 25% of the cocktail was applied after a sample was collected on Day 7 and the last 25% was applied on Day 14 after sample was collected for TPH and THC. The treated spike soil was thoroughly mixed or agitated after each application and sprinkle with water every day. Other samples were collected on Day 21 and Day 56 from the first day of treatment. The TPH and THC results were fed back to the RSM-DX program as actual results, which the model used to predict an optimized formulation for the bioremediation cocktail.