In Vitro Dissolution Studies
Drug release from the film was investigated through dissolution studies; those were conducted using 100 mL of buffer pH 6.8 solution as a dissolution media. A paddle apparatus was used for this study, and dissolution media was heated to 37°C using a magnetic stirrer and stirred at 100 rpm. A 1 cm × 1 cm film section was weighed and placed in dissolution media. Film samples with a weight of approximately 0.03 g were used. At set time intervals, 10 mL was drawn out and replaced with 10 mL of same dissolution media at the same temperature. Time intervals used were 0, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 60, 120, and 180 min. Solutions were then analyzed spectrophotometrically at 263 nm. Three replicates were carried out for dissolution of F5 and F6 films, and an average was calculated. Same dissolution method was used to carry out dissolution studies for ibuprofen sodium alone. Weight of ibuprofen sodium used was approximately 0.002 g. Statistical analysis was performed using two-way ANOVA to calculate the p value.