Chloroquine was shown to impair early stages of virus replication by interfering with the pH-dependent endosome-mediated viral entry of several different viruses [28]. Due to the alkalization of endosomes, chloroquine was an effective in vitro treatment against Chikungunya virus when added to Vero cells prior to virus exposure. A pH-dependent mechanism of entry of coronavirus into target cells was also reported for SARS-CoV-1 [29]. The activation step that occurs in endosomes at acidic pH results in fusion of the viral and endosomal membranes, leading to the release of the viral SARS-CoV-1 genome into the cytosol. In the absence of antiviral drug, the virus is targeted to the lysosomal compartment where the low pH, along with the action of enzymes, disrupts the viral particle, thus liberating the infectious nucleic acid and, in several cases, enzymes necessary for its replication. Hence, the lysosomal space has been proposed as a major target site to tackle SARS-CoV-2 [30].