A. Original dot plots of CD45+CD19+, CD45+CD11c+, CD45+CD3+ CD4+, CD45+CD3+ CD8+ and CD45+CD11b+ F4/80+ cells recruited to spleens of mice, which were untreated (control, upper panels) and treated with 1000 mg/l nCu (lower panels). Numbers depict the percent of cells in the respective quadrants, acquired within the dead cell gate