Fig. 6.  Intracellular glucose concentration calculated from Förster resonance energy transfer ratio dose-response curves. A: calculated intracellular glucose concentration in H441 cells grown on plastic and exposed to 5 mM (●) or 15 mM d-glucose (15 mM; hyperglycemia, ▲) or exposed to the same conditions in the presence of 3-bromopyruvic acid (BrPy; ○ or △, respectively). Values were calculated using the dose-response curve shown in Fig. 4D. Individual data points are shown with mean ± SD; n = 117 individual cells. ****P < 0.0001 between groups as indicated. B: calculated intracellular glucose concentration for H441 cells grown at air-liquid interface in either 5 mM glucose (●), 15 mM glucose (▲), or 5 mM glucose in the presence of BrPy (○). Individual data points are shown with mean ± SD; n = 83 individual cells. ****P < 0.0001 between groups as indicated. C: calculated intracellular glucose for human bronchial epithelial cells (HBECs) cultured at air-liquid interface in either 5 mM glucose (●) or 15 mM glucose (▲). Individual data points are shown with mean ± SD; n = 150 individual cells. ****P < 0.0001 between groups as indicated.