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PMC:64779 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, Al 1 1874 382 show
1 Background Opitz GBBB syndrome (OS; Opitz syndrome) is a genetically and phenotypically complex disorder define 1 4032 0 show
2 Results Yeast two-hybrid screens identify Alpha 4 as an interacting partner of both MID1 and MID2 In order t 1 3391 0 show
3 Results MID1 and MID2 tether Alpha 4 to the microtubules To investigate whether Alpha 4 also associated with 1 1253 0 show
4 Results Alpha 4 does not co-localise with MID1 or MID2 proteins harboring in-frame B-box deletions Both endo 1 4480 0 show
5 Results The coiled-coil domains of MID1 & MID2 are required for homodimerisation and microtubule binding but 1 1761 0 show
6 Results MID1 & MID2 can form heterodimers on microtubules Given their high level of identity, we investigate 1 1139 0 show
7 Results The B-boxes of MID1 & MID2 are sufficient to bind Alpha 4 To verify that the Alpha 4 interaction and 1 1830 0 show
8 Results MID1 is phosphorylated on serine and threonine residues As Alpha 4 is a regulator of PP2-type serine 1 1607 0 show
9 Results Identification of potential sites of phosphorylation in MID1 and MID2 Computer prediction of potenti 1 1176 0 show
10 Discussion We have previously shown that the X-linked form of Opitz GBBB syndrome (OS) results from loss of fun 1 11161 0 show
11 Conclusions The finding that Alpha 4, a rapamycin-sensitive regulatory subunit of PP2-type phosphatases, interac 1 483 0 show
12 Materials & Methods Miscellaneous enzymes and chemicals All restriction endonucleases were purchased from New England Bi 1 469 0 show
13 Materials & Methods The yeast two-hybrid screen The ProQuest™ yeast two-hybrid system (Invitrogen, Mulgrave, NSW) was em 1 3034 0 show
14 Materials & Methods Generation of GFP- and myc-full-length cDNA fusion constructs for immunofluorescence The generation 1 1096 0 show
15 Materials & Methods Generation of MID1 and MID2 domain-specific deletion constructs for immunofluorescence and yeast two 1 2333 0 show
16 Materials & Methods Transfection and immunofluorescence analysis of GFP-MID1 constructs Preparations of the various GFP- 1 1511 0 show
17 Materials & Methods Immunoprecipitation and western analysis Preparations of the various GFP- and myc-tagged expression 1 2036 0 show
18 Materials & Methods Computer-assisted detection of serine/threonine phosphorylaton sites Analysis of putative consensus 1 646 0 show
19 Materials & Methods Note Added In Proof During the review of this manuscript, a paper by Trockenbacher et al [Trockenbac 1 620 0 show
20 Caption-Figure 1 Alpha 4 interacts with MID1 and MID2. (A) Yeast two-hybrid analysis of the interaction between MID1 1 1103 0 show
21 Caption-Figure 2 MID1/Alpha 4 and MID2/Alpha 4 interactions are maintained in all MID domain-specific deletions excep 1 1073 0 show
22 Caption-Figure 3 MID1 and MID2 can homo- and heterodimerise with one another. (A) Yeast two-hybrid assay for MID1 and 1 1056 0 show
23 Caption-Figure 4 The B-boxes of MID1 are sufficient to bind Alpha 4. (A) Yeast two-hybrid analysis shows that the MID 1 852 0 show
24 Caption-Figure 5 MID1 contains phosphorylated serine and threonine residues. (A) Extracts from untransfected Cos1 cel 1 1988 0 show
25 Caption-Table 1 The MID1 and MID2 deletion constructs used in pEGFP-C2 for cellular co-localisation analysis, co-imm 1 381 0 show