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PMC:6333292 JSONTXT

Divisions (31) JSON TSV

div. section text # proj. length # Ann.
0 TIAB Identification of CMTM6 and CMTM4 as PD-L1 protein regulators The clinical benefit in patients with 1 1587 506 show
1 Body Antibodies that block the PD-1 – PD-L1 axis are currently evaluated in approximately 800 clinical st 1 14056 0 show
2 Methods Cell lines A375, DLD1, RKO, H2030, and H2122 cells were purchased from American Type Culture Collect 1 1128 0 show
3 Methods Identification of genetic regulators The approach as described in Brockmann et al. was followed to i 1 2608 0 show
4 Methods Generation of knockout cell lines Knockout cell lines were generated using the CRISPR/Cas9 system. T 1 1446 0 show
5 Methods Immunoprecipitation, SDS-PAGE and Western blot analysis Cells for Western blot analysis were seeded 1 1732 0 show
6 Methods Pulse Chase and EndoH-PNGaseF treatment V5-tagged PD-L1 transduced CMTM6 overexpressing A375 cells, 1 1129 0 show
7 Methods Viral vectors Lentiviral shRNA vectors were retrieved from the arrayed TRC human genome-wide shRNA c 1 2582 0 show
8 Methods Antibodies The following antibodies were used for Western Blot analyses and immunoprecipitations: an 1 1261 0 show
9 Methods RNA isolation, first strand cDNA synthesis and qRT-PCR Total RNA was isolated from cells using the R 1 642 0 show
10 Methods IL-2 production assay Human peripheral blood T cells (Sanquin) were activated and transduced with a 1 1071 0 show
11 Methods Membrane fractionation and MS analysis Snap-frozen cell pellets were lysed by gentle homogenization 1 1325 0 show
12 Methods RP-nanoLC-MS/MS Proteomics data were acquired using an UHPLC 1290 system (Agilent) coupled to an Orb 1 971 0 show
13 Methods Database search Raw files were processed using MaxQuant version 1.5.3.30 and searched against the hu 1 531 0 show
14 Methods Analysis of CMTM6 RNA levels and correlation between CMTM6 and CD274 RNA levels in TCGA samples TCGA 1 1146 0 show
15 Methods Immunohistochemistry Immunohistochemistry of the formalin-fixed paraffin-embedded samples was perfor 1 1095 0 show
16 Methods Statistical analysis All student T tests were two tailed under the assumption of equal variance betw 1 112 0 show
18 Caption-Extended data Figure 1 PD-L1 is regulated by IFNγ and by the UTR in HAP1 (a) Flow cytometric analysis of PD-L1 expression o 1 1139 0 show
19 Caption-Extended data Figure 2 RNA expression of CMTM6 in human cancers and correlation with PD-L1 mRNA levels. Pearson correlation 1 1654 0 show
20 Caption-Extended data Figure 3 Regulation of PD-L1 by CMTM6 in different tumor types. Flow cytometric analysis of PD-L1 expression 1 2055 0 show
21 Caption-Extended data Figure 4 CMTM4 and CMTM6, but not other CMTM family members, are regulators of PD-L1. (a) Validation of CMTM6 1 1780 0 show
22 Caption-Extended data Figure 5 CMTM6 downregulation does not affect MHC class I and PD-L2 cell surface levels or PD-L1 mRNA levels 1 1597 0 show
23 Caption-Extended data Figure 6 Specificity and membrane localization of CMTM6, and co-localization with PD-L1 in human tumors. (a), 1 1021 0 show
24 Caption-Extended data Figure 7 Interactions between CMTM6, PD-L1, and CMTM4, and effect of CMTM6 on PD-L1 stability. (a) A375 paren 1 1582 0 show
25 Caption-Extended data Figure 8 Aspects of PD-L1 regulation by CMTM6 and STUB1. (a) V5-tagged PD-L1 was introduced into parental, CM 1 1514 0 show
26 Caption-Extended data Figure 9 Orientation mapping of CMTM6. (a) Predicted domain topology of CMTM6 according to TMHMM Server v. 2. 1 396 0 show
27 Caption-Extended data Figure 10 Selectivity of CMTM6 and CMTM6 loss alleviates PD-L1-mediated T cell suppression. (a) Comparative me 1 1393 0 show
28 Caption-Figure 1 Identification of CMTM6 as a modulator of PD-L1 expression. (a) Flow cytometry-based screen for modu 1 1300 0 show
29 Caption-Figure 2 CMTM6 regulates PD-L1 expression in different tumor types and primary dendritic cells. (a,b,f) Relat 1 1277 0 show
30 Caption-Figure 3 Identification of CMTM4 as a second PD-L1 regulator. (a) Haploid genetic screen for modulators of PD 1 798 0 show
31 Caption-Figure 4 CMTM6 forms a molecular partner of PD-L1 and regulates PD-L1 protein stability. Time course of PD-L1 1 1517 0 show