Figure 7 Histone Mark Response to DNA Damage with PARP Inhibition and Persistent Ser-ADPr
(A) 293T cells were pretreated with DMSO or olaparib and treated with H2O2. Western blotting analysis of the changes in histone H3 K9ac, K9me3, S10P, K14ac, K27ac, K27me3, and K36ac, as well as total pan-Kac histone acetylation and cell-cycle protein levels was performed at the indicated times after the induction of DNA damage.
(B) U2OS WT and ARH3 KO cells were treated with H2O2. The levels of H3K9ac, H3K9me3, and pan-Kac were examined by western blotting at the indicated time points.