Figure 3 Canonical H3 Histone Marks Reduce the Efficiency of H3S10ADPr on H3 Peptide
(A) Autoradiogram showing PARP1/2 + HPF1-mediated ADPr of H3 peptide with WT, K9ac, K9me1, and S10ph modifications. Coomassie staining of the SDS-PAGE is included.
(B) As in (A), except PARP1 and HPF1 only, with H3 (1–20aa) WT, K9ac, K9me1, K9me2, and K9me3 peptides. Coomassie staining of the SDS-PAGE is included.
(C) As in (B), except with H3 (1–20aa) WT, K4ac, K4me3, K9ac, K9me1, K9me3, S10ph, K14ac, K18ac, and K18me3 peptides. Coomassie staining of the SDS-PAGE is included.
(D) As in (B), except with H3 (21–44aa) WT, K27ac, K27me1, K27me2, K27me3, and WT peptides. Coomassie staining of the SDS-PAGE is included.