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PMC:6120343 JSONTXT

Divisions (29) JSON TSV

div. section text # proj. length # Ann.
0 TIAB Structural Basis of Mitochondrial Receptor Binding and Constriction by DRP1 Summary Mitochondrial in 1 1208 309 show
1 Introduction Fragmentation of the mitochondrial reticulum disperses units of the organelle during cell division1, 1 3507 0 show
2 Results and Discussion To date, many structural studies of dynamin-family proteins have relied upon mutated or truncated co 1 1048 0 show
3 Results and Discussion Structure of the DRP1-MID49 cofilament Incubating equimolar ratios of DRP1 with soluble MID49126-454 1 1507 0 show
4 Results and Discussion Structure-based mutants disrupt DRP1-MID49 assembly MID49’s DRR motif occupied the space between two 1 3503 0 show
5 Results and Discussion Nucleotide-induced conformational changes enable receptor binding Understanding the allosteric coupl 1 1332 0 show
6 Results and Discussion GTP-hydrolysis induces filaments to curl into rings We next evaluated the dynamics of the DRP1+MID49 1 1158 0 show
7 Results and Discussion A model for closed DRP1 rings In a separate but related experiment, we also evaluated the assembly p 1 2675 0 show
8 Discussion We note that with an inner diameter of ~16nm, the closed ring may be sufficient to sever a double-me 1 2287 0 show
9 Methods Construct design Wild type (WT) DRP1 isoform 2 sequence was purchased from DNASU (sequence ID HsCD00 1 746 0 show
10 Methods Protein purification Protein purification was performed as described52. Briefly, plasmids containing 1 4571 0 show
11 Methods Filament assembly, EM sample preparation, data acquisition and processing To assemble DRP1-MID49126- 1 4788 0 show
12 Methods Liposome and Nanotube reactions Liposomes were made as described before52. Briefly, 1,2-dioleoyl-sn- 1 959 0 show
13 Methods Model building The general procedure for atomic model interpretation and validation using Rosetta we 1 3634 0 show
15 Caption-Extended Data Figure 1 DRP1 and MID49 assembly states (a-j) DRP1 assembly states visualized with negative stain electron mi 1 454 0 show
16 Caption-Extended Data Figure 2 CryoEM and 3D Reconstruction (a) An electron cryo-micrograph of DRP1-MID49126-454 filaments formed w 1 687 0 show
17 Caption-Extended Data Figure 3 Intra- and Inter-filament Interactions (a) The triangular structure seen in cross section. ‘Side 1’ 1 1150 0 show
18 Caption-Extended Data Figure 4 Resolution Estimates (a-b) Local resolution estimates computed by Resmap74. (a) Histogram of voxel v 1 351 0 show
19 Caption-Extended Data Figure 5 Rosetta-based Model Refinement (a): Complete assembly used for Rosetta-based model building with the 1 628 0 show
20 Caption-Extended Data figure 6 Map-to-Model Fits and Role of the L1NS and L2S loops Examples of models fit within B-factor sharpene 1 685 0 show
21 Caption-Extended Data Figure 7 Sequence Conservation and Key Interaction Sites (a) Multiple sequence alignment of the regions near 1 546 0 show
22 Caption-Extended Data Figure 8 Biochemical and Structural Characterization of Mutants (a-d) Size exclusion chromatography traces fo 1 723 0 show
23 Caption-Extended Data Figure 9 DRP1G362D Rings on Model Membranes (a) DOPS liposomes used in the study. (b) DOPS liposomes after in 1 474 0 show
24 Caption-Extended Data Figure 10 DRP1G362D forms 12-dimer closed rings (a) 2D class average of the rings. The red dashed circle indic 1 444 0 show
25 Caption-Figure 1 Architecture of the DRP1-MID49 linear filament (a) DRP1 and MID49 domain arrangements. (b) Density m 1 386 0 show
26 Caption-Figure 2 Key DRP1-MID49/51 receptor interfaces and regulatory phosphorylation site (a) Receptor interfaces 1 1 945 0 show
27 Caption-Figure 3 Nucleotide-driven allosteric elongation of DRP1 exposes MID49/51 receptor binding sites (a) nucleoti 1 873 0 show
28 Caption-Figure 4 Dynamic instability of the DRP1-MID49 linear assembly and curling into closed DRP1 rings (a) Three-s 1 468 0 show
29 Caption-Figure 5 Drp1G362D cannot bind MID49 and forms rings exclusively with GMPPCP or GTP (a) CryoEM micrograph of 1 679 0 show