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PMC:6093860 JSONTXT

Divisions (19) JSON TSV

div. section text # proj. length # Ann.
0 TIAB The first transmembrane region of complement component-9 acts as a brake on its self-assembly Comple 1 1068 188 show
1 Introduction The MAC represents the terminal portion of the complement system, and functions to form large pores 1 3845 0 show
2 Results The X-ray crystal structure of monomeric C9 The structure of C9 reveals that the four domains (Throm 1 2155 0 show
3 Results Mobility in TMH1 is essential for C9 self-association To investigate the hypothesis that TMH1 blocks 1 792 0 show
4 Results The cryo-EM structure of poly C9 To further investigate the structural transitions associated with C 1 1739 0 show
5 Results The molecular transitions that control MAC assembly A comparison to the polyC9 structure with the mo 1 1453 0 show
6 Discussion Previous data suggest that both TMH1 and TMH2 of C8 fully enter the membrane prior to recruitment of 1 1751 0 show
7 Methods Recombinant C9 purification Human C9 and mouse C9 protein were purified using similar methods (with 1 2078 0 show
8 Methods Murine C9 crystallisation The murine C9 gene was synthesised (GenScript) with three mutations (N28E; 1 1107 0 show
9 Methods X-ray data collection and model building The data were merged and processed using XDS24,25, POINTLES 1 2700 0 show
10 Methods PolyC9 preparation and data collection Mammalian cell expressed human C9 (with the two native N-glyc 1 1363 0 show
11 Methods Cryo-EM data processing Unless stated otherwise all processing was performed with RELION (v2.1b.1)43 1 1688 0 show
12 Methods PolyC9 model building and model validation Model building of the polyC9 was performed in COOT (0.8.8 1 1449 0 show
13 Methods Haemolytic assay Turbidity measurements were performed using sheep EAC1-8 prepared in DHB++ pH 7.4 ( 1 1226 0 show
14 Methods Data availability Data supporting the findings of this manuscript are available from the correspondi 1 292 0 show
15 Caption-Fig. 1 The X-ray crystal structure of complement component 9. a The X-ray structure of C9 shown in cartoon 1 822 0 show
16 Caption-Fig. 2 The C9 TMH1 movement is necessary for pore assembly. a Cartoon model of a C9 monomer (left) disulphi 1 1230 0 show
17 Caption-Fig. 3 C9 structure in the monomeric and assembled forms. a The cryo EM structure of polyC9 with 22 subunit 1 1075 0 show
18 Caption-Fig. 4 Schematic diagram of the unidirectional C9 assembly. We hypothesise that during the assembly, bindin 1 402 0 show