FIGURE 3:  DNA-damaging conditions result in phosphorylation of Ser-26 and -30 of FUS. (A) Lysates of H4 cells treated with increasing amounts of calicheamicin were analyzed by Western blotting with commercial FUS and anti-FUS(pSer26) or anti-FUS(pSer30) antibodies; n = 2. (B, C) Lysates of H4 cells subjected to DNA-damaging conditions (0.5 nM calicheamicin, 5 nM calyculin-A, 200 μM etoposide, 10 μM camptothecin, 2 μM doxorubicin, 100 nM BLM, 90 mJ UV, or 20 Gy IR) were analyzed by Western blotting with commercial FUS and anti-FUS(pSer26) or anti-FUS(pSer30) antibodies; n = 3 for each treatment except IR in which n = 2. (D) Lysates of H4 cells treated with and without DNA-PK inhibitor (NU7441) prior to calicheamicin treatment were analyzed by Western blotting with commercial FUS and anti-FUS(pSer26) or anti-FUS(pSer30) antibodies; n = 3.