> top > docs > PMC:6085830 > divs

PMC:6085830 JSONTXT

Divisions (20) JSON TSV

div. section text # proj. length # Ann.
0 TIAB The prionlike domain of FUS is multiphosphorylated following DNA damage without altering nuclear loc 1 1642 376 show
1 INTRODUCTION FUS (fused in sarcoma) is a ubiquitously expressed DNA/RNA-binding protein implicated in diverse fun 1 5222 0 show
2 RESULTS The FUS prionlike domain is phosphorylated at numerous serines and threonines following calicheamici 1 3115 0 show
3 RESULTS Ser-26 and -30 in the FUS prionlike domain are phosphorylated following DNA-damaging stress Of the t 1 4599 0 show
4 RESULTS FUS remains nuclear following PrLD phosphorylation induced by DNA damage It was previously suggested 1 3176 0 show
5 RESULTS DNA-PK–dependent phosphorylation of the PrLD does not promote FUS cytoplasmic localization We asked 1 3379 0 show
6 DISCUSSION The results of our mass spectrometry experiments suggest that extensive FUS PrLD phosphorylation can 1 8445 0 show
7 MATERIALS AND METHODS Cell culture H4 neuroglioma (ATCC HTB-148) and HEK293T (ATCC CRL-11268) cells were cultured in DMEM- 1 1564 0 show
8 MATERIALS AND METHODS Western blotting and immunoprecipitation Cell lysates were mixed with 4X NuPAGE LDS Sample Buffer (T 1 1459 0 show
9 MATERIALS AND METHODS Immunocytochemistry and ImageJ quantification Cells were grown on glass coverslips for 24 h prior to 1 1757 0 show
10 MATERIALS AND METHODS Phosphatase treatment of FUS protein Immunoprecipitated FUS was treated with calf intestinal phospha 1 471 0 show
11 MATERIALS AND METHODS Plasmids, knockdown, and mammalian cell transfection Wild-type FUS and its phosphomimetic variants w 1 964 0 show
12 MATERIALS AND METHODS Custom peptide and antibody production and testing Pairs of phosphorylated and nonphosphorylated pep 1 1095 0 show
13 MATERIALS AND METHODS Mass spectrometry H4 cells were treated with 50 nM calicheamicin or 30 µM camptothecin for 1 h at 37 1 955 0 show
14 Caption-FIGURE 1: The prionlike domain of cellular FUS contains numerous phosphorylation sites. (A) Lysates from multi 1 1148 0 show
15 Caption-FIGURE 2: Custom anti-FUS(pSer26) and anti-FUS(pSer30) antibodies are specific to phosphorylated FUS. (A, B) L 1 1131 0 show
16 Caption-FIGURE 3: DNA-damaging conditions result in phosphorylation of Ser-26 and -30 of FUS. (A) Lysates of H4 cells 1 838 0 show
17 Caption-FIGURE 4: FUS remains nuclear following DNA-PK–dependent phosphorylation at Ser-30. (A) H4 cells were fixed fo 1 891 0 show
18 Caption-FIGURE 5: Calicheamicin-induced phosphorylated FUS is maintained in the nucleus following osmotic stress. (A) 1 1184 0 show
19 Caption-FIGURE 6: Phosphorylation of the FUS prionlike domain is not linked to cytoplasmic localization. (A) H4 cells 1 987 0 show