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PMC:56590 JSONTXT

Divisions (13) JSON TSV

div. section text # proj. length # Ann.
0 TIAB Itt1p, a novel protein inhibiting translation termination inSaccharomyces cerevisiae Background Term 0 1373 0 show
1 Background The final step of protein biosynthesis represents the termination codon-dependent release of a nasce 0 4182 0 show
2 Results Isolation and characterization of the ITT1 gene S. cerevisiae strain 2G-DM8, carrying the non-suppre 0 3286 0 show
3 Results Itt1p interacts with eRF1 and eRF3 The inhibition of translation termination by excess Itt1p could b 0 1686 0 show
4 Results The suppressor effect of excess Itt1p depends on both eRF1 and eRF3 release factors To test the role 0 2027 0 show
5 Discussion This paper describes a novel protein, Itt1p, involved in the control of translation termination in y 0 5859 0 show
6 Conclusions The data presented in this work show that the increased expression of the ITT1 (YML068w) gene reduce 0 876 0 show
7 Materials and Methods Genetic methods We used standard organic (YEPD) and synthetic complete (SC) media for yeast [29] and 0 621 0 show
8 Materials and Methods Strains and plasmids The yeast strains 2G-DM8 (MATα ade2-144,717 pheA10 his7-1 lys2-l87 trp1-1 ura3- 0 4690 0 show
9 Materials and Methods β-Galactosidase assays of yeast strains transformed with the pUKC815/817/181/819 series vectors The 0 926 0 show
10 Materials and Methods Preparation of yeast cell lysates Yeast cultures were grown to OD600 of 1.5, harvested, washed in wa 0 372 0 show
11 Materials and Methods Purification of eRF1, eRF3 and eRF3 fragments and assay for interaction with immobilized GST-Itt1p T 0 872 0 show
12 Materials and Methods Protein gel electrophoresis and Western blot analysis Protein samples were separated on an SDS polya 0 427 0 show