Template  Primers  Product Length  Melting Temperature  Hot Measurement  Fidelity  n
18S  gag gtt cga aga cga tca ga (sense)  316 bp  83.2 ± 02°C  78°C  1.89 ± 0.02  17
tcg ctc cac caa cta aga ac (antisense)
KCNJ10  tgg tgt ggt gtg gta tct gg (sense)  411 bp  83.2 ± 02°C  78°C  1.86 ± 0.02  15
tga agc agt ttg cct gtc ac (antisense)
KCNQ1  ttt gtt cat ccc cat ctc ag (sense)  239 bp  82.5 ± 02°C  79°C  1.85 ± 0.02  17
gtt gct ggg tag gaa gag (antisense)
KCNQ4  ccc gga aac cct tct gtg tc (sense)  245 bp  83.2 ± 02°C  80°C  1.87 ± 0.01  17
aaa gat gag cac cag gaa cc (antisense)