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PMC:512295 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB Altered mRNA expression of genes related to nerve cell activity in the fracture callus of older rats 0 1814 0 show
1 Background Bone formation to bridge the fracture gap following skeletal fracture slows with age in both humans 0 2515 0 show
2 Methods Rats Intact female Sprague-Dawley rats (Harlan Sprague-Dawley, Inc., Indianapolis, IN) were purchase 0 448 0 show
3 Methods Surgery Intact female Sprague-Dawley rats at 6 (young), 26 (adult) or 52 (older) weeks of age, weigh 0 1544 0 show
4 Methods RNA Sample Preparation and Microarray Processing Samples were prepared as described in the Affymetri 0 1640 0 show
5 Methods Data Analysis The Rat U34A GeneChip Microarray has probe sets for over 8,700 rat genes. Most probe s 0 2226 0 show
6 Results Radiology In all young rats, bone bridged the fracture gap by four weeks after surgery. By six weeks 0 579 0 show
7 Results General results On each array, on average, 5,200 genes were scored as absent, and 3,300 as present. 0 880 0 show
8 Results Similar responses at all three ages Up-regulated nerve-related genes are shown in Table 1. Two examp 0 844 0 show
9 Results Defects in the older rats SOM cluster analysis identified three types of defects in the older rats. 0 1962 0 show
10 Results Present/Marginal/Absent calls For each gene for each array, the Microarray Suite software reported a 0 673 0 show
11 Discussion In this study, as in our earlier work [7,10-12], the time required to reach radiographic union after 0 9174 0 show
12 Conclusions In summary, most genes respond to bone fracture with altered mRNA gene expression, including genes r 0 398 0 show
13 Pre-publication history The pre-publication history for this paper can be accessed here: 0 64 0 show