Because this QTL confers such strong disease resistance, and it is located at the very end of the chromosome of a species with very close affinity to the B genome of cultivated peanut, it is unlikely to suffer strong linkage drag. Overall it seems like an ideal candidate for the introgression of rust resistance into cultivated peanut. To facilitate this, we developed multiple markers surrounding the locus Ah-280. Also, markers were developed around the locus AHGS1350, which is linked to two strong QTL. To do this, we identified SNPs between A. magna and A. ipaënsis around these loci. Because of the very high similarity of A. ipaënsis to the B genome of A. hypogaea, 19 of 22 KASP markers designed successfully distinguished the rust-resistant wild genotypes and their allotetraploid derivatives from A. ipaënsis and A. hypogaea. For nine of the assays, the A. ipaënsis and A. hypogaea genotypes clustered together; for the other eight, the clusters of the A. hypogaea were shifted to an intermediate position because of the interfering signal generated from the DNA bases on the homeologous A-genome (as described in Bertioli et al. 2014). This distortion of clustering is easy to account for and does not affect the function of the markers.