The aim of the methods in this section was to develop reliable and easy-to-use DNA markers for the genomic region in A. magna K 30097 that confers rust resistance. Although A. magna K 30097 was of primary interest for this study, A. batizocoi K9484 also is being used in our research and for introgression in breeding programs (Leal-Bertioli et al. 2014b). Therefore, we aimed to develop markers that would function for both these species (Leal-Bertioli et al. 2014a,b).