Figure S4 shows a genome-wide comparison for the distribution of significant CSS peaks between the original results (Figure 2A) and three additional analyses—performed to evaluate composition of European cohorts given the variable sample sizes of cattle breeds. Overall, high concordance was observed between the significant peaks in cohort-wise analyses using all available individuals (original), with a maximum of 20 and 10 individuals per breed. For comparison, the significant regions were considered valid if found within the top 1% significant threshold. Notably, the significant regions detected in original analyses (Table S5) were found above the top 1%, with the only exception being a region on BTA-19 in the European large cohort being not detected with a maximum of 10 individuals per breed. Overall, the impact of varying the number of animals per breed had negligible impact on the CSS profile. Hence, we propose that the maximum number of animals should be used when possible.