The delay in colony formation in the sea3∆ mutant BIR assay strain suggested that sea3∆ mutants may have a delay in recovery post-DNA damage. Recovery is typically defined as resumption of mitosis after repair is completed and the checkpoint is turned off; recovery mutants demonstrate sustained activation of the DDR despite repair of the DSB (Vaze et al. 2002). A variety of proteins have been implicated in recovery and most are associated with the DNA damage checkpoint or repair (Guillemain et al. 2007; Leroy et al. 2003; Vaze et al. 2002).