Figure 4  mua-3 mutants showed temperature-dependent phenotypes. (A) mua-3(uy19) mutants do not show lethality at 15°. (B) Increase of temperature to 20° reduces survival of mua-3(uy19) mutants. (C and D) Another allele of mua-3 mutants shows temperature-dependent sterility. mua-3(rh195) mutants show a normal gonad morphology at 20° (A, white arrow) but become sterile due to detachment of the gonad at 25° (D, red arrows). (E) The sterility induced by high temperature was quantified by counting the progeny of mua-3 mutants and wild-type animals grown at 20° and 25°. ***P < 0.001 for comparison between the numbers of progeny of mua-3 at 20° and 25° by Student's t test.