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mua-3(uy19) were collected at the L4 stage and were randomly mutagenized with ethyl methanesulfonate (EMS) (Brenner 1974). Po animals were plated onto E. coli HB101 seeded plates and moved to 15°. F1 progeny were synchronized and remained at 15°. The homozygous F2 generation was synchronized and moved to 25°. The surviving animals were isolated.