Table 3 Gene Ontology terms identified by Blast2GO analysis for contigs significantly upregulated at 25° (FDR 0.1, P < 0.01, >2-fold change) GO Category GO ID Name GO ID Name # Contigs Biological Process GO:0071840 Cellular component organization or biogenesis GO:0016043 Cellular component organization 124 GO:0007005 Mitochondrion organization 18 GO:0006996 Organelle organization 50 GO:0009987 Cellular process GO:0007154 Cell communication 11 GO:0007049 Cell cycle 87 GO:0008219 Cell death 129 GO:0019725 Cellular homeostasis 34 GO:0006464 Cellular protein modification process 175 GO:0007010 Cytoskeleton organization 85 GO:0007165 Signal transduction 337 GO:0006412 Translation 27 GO:0032502 Developmental process GO:0009653 Anatomical structure morphogenesis 202 GO:0030154 Cell differentiation 152 GO:0009790 Embryo development 87 GO:0007275 Multicellular organismal development 315 GO:0040007 Growth GO:0016049 Cell growth 21 GO:0008152 Metabolic process GO:0009058 Biosynthetic process 155 GO:0005975 Carbohydrate metabolic process 27 GO:0009056 Catabolic process 170 GO:0006259 DNA metabolic process 43 GO:0006091 Generation of precursor metabolites and energy 11 GO:0006629 Lipid metabolic process 68 GO:0006139 Nucleobase-containing compound metabolic process 127 GO:0044238 Primary metabolic process 6 GO:0019538 Protein metabolic process 216 GO:0019748 Secondary metabolic process 6 GO:0051704 Multi-organism process GO:0044403 Symbiosis, encompassing mutualism through parasitism 20 GO:0016032 Viral process 20 GO:0050789 Regulation of biological process GO:0040029 Regulation of gene expression, epigenetic 4 GO:0000003 Reproduction 91 GO:0050896 Response to stimulus GO:0007610 Behavior 33 GO:0009628 Response to abiotic stimulus 51 GO:0009607 Response to biotic stimulus 83 GO:0009719 Response to endogenous stimulus 46 GO:0009605 Response to external stimulus 100 GO:0006950 Response to stress 237 GO:0044699 Single-organism process GO:0008283 Cell proliferation 91 GO:0008037 Cell recognition 26 GO:0007267 Cell–cell signaling 18 GO:0006810 Transport GO:0006811 Ion transport 52 GO:0015031 Protein transport 66 Molecular Function GO:0016209 Antioxidant activity 7 GO:0005488 Binding GO:0003779 Actin binding 34 GO:0005509 Calcium ion binding 51 GO:0030246 Carbohydrate binding 11 GO:0003682 Chromatin binding 22 GO:0008092 Cytoskeletal protein binding 20 GO:0003677 DNA binding 142 GO:1901363 Heterocyclic compound binding 1 GO:0008289 Lipid binding 33 GO:0003676 Nucleic acid binding 48 GO:0000166 Nucleotide binding 262 GO:0097159 Organic cyclic compound binding 1 GO:0005515 Protein binding 498 GO:0005102 Receptor binding 83 GO:0003723 RNA binding 42 GO:0008135 Translation factor activity, nucleic acid binding 5 GO:0003824 Catalytic activity GO:0016787 Hydrolase activity 98 GO:0016301 Kinase activity 15 GO:0003774 Motor activity 2 GO:0004518 Nuclease activity 6 GO:0008233 Peptidase activity 46 GO:0004721 Phosphoprotein phosphatase activity 26 GO:0004672 Protein kinase activity 58 GO:0016740 Transferase activity 89 GO:0009055 Electron carrier activity 19 GO:0030234 Enzyme regulator activity 66 GO:0060089 Molecular transducer activity GO:0004871 Signal transducer activity 13 GO:0001071 Nucleic acid binding transcription factor activity GO:0003700 Sequence-specific DNA binding transcription factor activity 95 GO:0004872 Receptor activity 43 GO:0005198 Structural molecule activity 10 GO:0005215 Transporter activity GO:0005216 Ion channel activity 6 Cellular Component GO:0030313 Cell envelope 1 GO:0071944 Cell periphery GO:0030312 External encapsulating structure 1 GO:0005886 Plasma membrane 134 GO:0005737 Cytoplasm GO:0016023 Cytoplasmic membrane-bounded vesicle 68 GO:0005829 Cytosol 134 GO:0005811 Lipid particle 9 GO:0005576 Extracellular region GO:0005615 Extracellular space 38 GO:0005578 Proteinaceous extracellular matrix 22 GO:0043226 Organelle GO:0005694 Chromosome 37 GO:0005929 Cilium 4 GO:0005856 Cytoskeleton 82 GO:0005783 Endoplasmic reticulum 115 GO:0005768 Endosome 19 GO:0072546 ER membrane protein complex 1 GO:0005794 Golgi apparatus 48 GO:0005764 Lysosome 6 GO:0005815 Microtubule organizing center 32 GO:0005739 Mitochondrion 78 GO:0000228 Nuclear chromosome 20 GO:0005635 Nuclear envelope 14 GO:0005730 Nucleolus 86 GO:0005654 Nucleoplasm 166 GO:0005634 Nucleus 233 GO:0005777 Peroxisome 3 GO:0005840 Ribosome 8 GO:0005773 Vacuole 5 GO:0043234 Protein complex 290 The three GO categories, Biological Process, Molecular Function, and Cellular Component, are presented along with GO terms of children and number of contigs assigned each term.