12°  15°  18°  21°  25°
Raw sequences  192,559,160  163,018,834  189,541,778  178,501,904  161,662,008
Sequences after QC filtering  182,390,970  154,294,234  179,245,463  167,554,816  152,818,985
DE contigs upregulated   4  104  697  3976
Upregulated contigs annotated by BLAST   1 (25%)  55 (53%)  354 (51%)  1902 (48%)
Upregulated contigs assigned GO terms   1 (25%)  54 (52%)  322 (46%)  1608 (40%)
Upregulated contigs assigned Enzyme Codes   0  5 (5%)  70 (10%)  487 (12%)
Upregulated contigs annotated by InterProScan   1 (25%)  50 (48%)  347 (50%)  1834 (46%)
Upregulated KEGG pathways   0  2  13  56
Upregulated KEGG Pathways with >2 enzymes   0  0  5  25
DE contigs downregulated   5  16  101  6166
Downregulated contigs annotated by BLAST   2 (40%)  5 (31%)  40 (40%)  3481 (56%)
Downregulated contigs assigned GO terms   2 (40%)  3 (12%)  31 (31%)  2972 (48%)
Downregulated contigs assigned Enzyme Codes   0  0  8 (8%)  626 (10%)
Downregulated contigs annotated by InterProScan   2 (40%)  9 (56%)  45 (45%)  —
Downregulated KEGG pathways   0  0  1  —
Downregulated KEGG pathways with >2 enzymes   0  0  0  —