The other three heteroplasmic sites occurred in the anticodon of putative tRNA genes (positions 9309, 11755, and 12160), and each allele occurred at a frequency of approximately 0.5 in all three cases. Interestingly, they appear to be orthologous to the three confirmed heteroplasmic sites in T. rathkei tRNA anticodons. The heteroplasmy at position 12160 has previously been reported to be widespread among terrestrial isopods, including C. convexus (Doublet et al. 2008). The heteroplasmies at positions 9309 and 11755 were confirmed by Sanger sequencing with the original DNA pool used for 454 sequencing, as well as a set of 12 individual samples originating from diverse geographic locations (Figure S3).