Finally, there were two additional putative heteroplasmic sites present at high frequencies in only one of the two individuals. One of these, unique to the female individual, was in a noncoding region (position 10074). The other was not a substitution, but a single base-pair deletion causing a frame shift within the cox3 gene (position 11244). Interestingly, the alternate allele at this position was detected in the female sample, but at a very low frequency (4%).