Table 1  Mitochondrial sequence heteroplasmies in Trachelipus rathkei
Pos.  Ref. Allele  Alt. Allele  Alt. Freq., M  Cov., M  Alt. Freq., F  Cov., F  Notes
62  T  A  0.45  252  0.39  111  Noncoding palindrome sequence
73  T  A  0.47  299  0.39  127  Noncoding palindrome sequence
9346*  G  A  0.53  1740  0.51  718  tRNA Leu1/Leu2 anticodon shared with C. convexus
10074  G  A  0  1737  0.47  657  Noncoding
11244  A  —  0.39  1726  0.04  756  cox3 frameshift, affecting amino acids 92 – 277; creates premature stop codon at position 106
11785*  G  C  0.58  1594  0.49  651  tRNA Arg/Gly anticodon shared with C. convexus; also causes cox3 substitution (E273Q)
12188*  A  G  0.41  1638  0.47  629  tRNA Ala/Val anticodon shared with C. convexus
14107  T  A  0.5  193  0.46  74  Noncoding palindrome sequence
Alt. freq., Cov. M, and Cov. F indicate the frequency of the alternative allele in the sequence reads, and the total coverage at that site, in the male and female samples, respectively. The — at site 11244 indicates that the alternate allele is a 1-bp deletion. *Heteroplasmic sites that were confirmed by Sanger sequencing.