NFAT Luciferase assay
HEK293 cells were transfected with indicated plasmids in combination with 10 ng TK Renilla, test and/or empty vector, and 50 ng NFAT-Luc (Stratagene Inc). Transfections were carried out in 96 well format using approximately 25,000 cells per well. Cells were exposed to either DMSO, 5 nM CsA, 1 μM PMA for 8 hours. Reporter activities were determined 48 hours after transfection with the Dual-Glo luciferase reagent as per manufacturer's instructions (Promega).