Figure 3  The relative proportional subpopulation distribution of protoplast expressing sGFP. The fluorescence intensity was partitioned into P1, P2, and P3 subpopulations (P1: 103–104; P2: 104–105; P3: >105). The total percentage of protoplasts expressing sGFP on FCA is shown by the sum of percentages of transfected cells on each subpopulation. The circular charts above each bar graph show the ratio of transfected cells on each subpopulation. The transfected protoplast proportion of each subpopulations relative to all transfected protoplasts with a fluorescence intensity exceeding a threshold value (103) was calculated on FCA. The error bars show the SEM (standard error of the mean) for the data acquired from three independent transfections. Mock samples were prepared by PEG-transfection with no plasmid DNA.