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PMC:3702119 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB Assessment of PotentialIn vitroGenotoxic and Cytotoxic Effects of Bupropion Hydrochloride (Wellbutri 2 1256 29 show
1 INTRODUCTION Depression is a state of mental illness and has been portrayed in different ways; in the 20th centur 2 2626 0 show
2 MATERIALS AND METHODS Test drug and chemicals WB (bupropion hydrochloride, C13 H18 ClNOHCl, Glaxo Smith Kline, USA is an a 2 632 0 show
3 MATERIALS AND METHODS Donors and collection of blood samples The study was carried out by using blood samples from four he 2 725 6 show
4 MATERIALS AND METHODS Lymphocyte cultures Venous blood was drawn in sterile condition from each subject and whole culture 2 2623 80 show
5 MATERIALS AND METHODS Alkaline single-cell gel electrophoresis (comet assay) Comet assay detects DNA damage through electr 2 766 0 show
6 MATERIALS AND METHODS Cell culture and cellular viability determination The cell line human cortical neural (HCN2) (Americ 2 1381 60 show
7 MATERIALS AND METHODS Sister chromatid exchange analysis For SCE analysis, Bromodeoxy uridine (BrdU; Sigma-Aldrich, India) 2 566 0 show
8 MATERIALS AND METHODS Cytokinesis-block micronucleus assay The CBMN assay was carried out using techniques of Fenech.[17] 2 418 0 show
9 MATERIALS AND METHODS Slide scoring and statistical analysis For SCE analysis, a minimum of 50 well spread metaphases were 2 905 0 show
10 RESULTS Deoxyribonucleic acid damage analysis Figure 1b summarizes the induction of WB mediated effects in h 2 659 0 show
11 RESULTS Sister chromatid exchange analysis SCEs were significantly induced by WB in human blood cultures [Fi 2 596 0 show
12 RESULTS Micronucleus test Figure 3a–i summarizes results of the MN assay. WB treatment of blood samples at t 2 1586 0 show
13 RESULTS Cytotoxicity assays The human cortical neuronal cells were treated with WB. In order to determine th 2 1209 0 show
14 DISCUSSION Our results clearly indicate that WB induces DNA damage in human blood cells and that WB-induced DNA 2 6427 132 show
15 Caption-Figure 1 (a) Chemical structure of wellbutrin (WB) (b) Induction of deoxyribonucleic acid (DNA) damage by WB 2 632 0 show
16 Caption-Figure 2 Induction of sister chromatid exchange (a) Metaphase showing SCE treated by 0 μM wellbutrin (WB) and 2 351 0 show
17 Caption-Figure 3 Cells treated with wellbutrin (WB) at (a) start of the cultures (b) after 24 h and (c) after 44 h (d 2 419 0 show
18 Caption-Figure 4 Loss of neuritis in human cortical neural 2 cells treated with wellbutrin. Cells were differentiated 2 352 0 show