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PMC:3317373 JSONTXT

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div. section text # proj. size # Ann.
0 TIAB The Absence of Nrf2 Enhances NF-κB-Dependent Inflammation following Scratch Injury in Mouse Primary 33 1279 605 show
1 1. Introduction Brain damage following traumatic injury is a result of direct (primary injury) and indirect (seconda 26 1544 613 show
2 2. Methods and Materials 2.1. Primary Culture and Identification of Mouse Astrocytes Nrf2 knockout ICR mice were kindly provi 26 1428 594 show
3 2. Methods and Materials 2.2. In Vitro Model of TBI Established by Scratch Injury Astrocyte scratch injury was performed as i 26 530 219 show
4 2. Methods and Materials 2.3. Cell Death Measurement by CCK-8 Assay 1 × 103/well cells were seeded to 96-well culture plates 26 613 276 show
5 2. Methods and Materials 2.4. Electrophoretic Mobility Shift Assay (EMSA) Monolayers of astrocytes were washed with PBS and h 26 1801 788 show
6 2. Methods and Materials 2.5. RT-PCR Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was s 26 598 273 show
7 2. Methods and Materials 2.6. Western Blot To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0 26 1506 667 show
8 2. Methods and Materials 2.7. Enzyme-Linked Immunosorbent Assay (ELISA) Cells of four groups were homogenized as mentioned ab 26 1171 334 show
9 2. Methods and Materials 2.8. Gelatine Zymography Cells of four groups were homogenized in lysis buffer containing 50 mM Tris 26 893 387 show
10 2. Methods and Materials 2.9. Statistical Analysis Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple c 26 222 86 show
11 3. Results 3.1. Depletion of Nrf2 Aggravated the Cell Death in Astrocytes after Scratch Injury Cell death was d 26 558 259 show
12 3. Results 3.2. Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury It 26 503 255 show
13 3. Results 3.3. Expression of Proinflammatory Cytokines Was Elevated in Nrf2 Knockout Astrocytes after Scratch 26 1726 1082 show
14 3. Results 3.4. Expression and Activity of MMP9 Were Greatly Enhanced in Nrf2 Knockout Astrocytes after Scratch 26 950 585 show
15 4. Discussion The present study demonstrated that scratch injury induced the upregulation of NF-κB DNA binding act 26 5308 3106 show