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PMC:3312845 JSONTXT

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div. section text # proj. size # Ann.
0 TIAB Tumor necrosis factor-α-mediated threonine 435 phosphorylation of p65 nuclear factor-κB subunit in e 33 1804 1006 show
1 Background Status epilepticus (SE) is a medical emergency with significant mortality [1]. SE has been defined a 26 4081 1951 show
2 Methods Experimental animals This study utilized progeny of Sprague-Dawley (SD) rats (male, 9 - 11 weeks old 26 791 291 show
3 Methods Intracerebroventricular drug infusion Rats were divided into two groups: vehicle (saline)-treated an 26 1432 691 show
4 Methods Seizure induction Three days after surgery, rats were treated with pilocarpine (380 mg/kg, i.p.; Sig 26 1163 469 show
5 Methods Tissue processing At designated time points (non-SE: 12 hr, 1 day, 2 days, 3 days, 4 days and 1 week 26 792 331 show
6 Methods Immunohistochemistry Free-floating sections were first incubated with 10% normal goat serum for 30 m 26 1521 624 show
7 Methods Double immunofluorescence study Sections were incubated with 3% bovine serum albumin in PBS for 30 m 26 1463 483 show
8 Methods Fluoro-Jade B staining Fluoro-Jade B (FJB) staining was used to identify degenerating neurons in tis 26 1222 515 show
9 Methods Volumetric analysis and cell counts To measure vasogenic edema, the volume of anti-rat IgG positive 26 2229 790 show
10 Methods Quantification of data The fluorescence intensities of SMI-71/p65-Thr435 phosphorylation or GFPA/p65 26 1281 501 show
11 Methods Data analysis Data obtained from volumetric analysis, cell counts, and quantitative measurements wer 26 335 126 show
12 Results TNF-α, and TNF receptor expression In non-SE induced animals of the saline-infused groups, TNF-α imm 26 948 546 show
13 Results Effect of sTNFp55R infusion on SE-induced serum-protein extravasation and neuronal damage In our pre 26 967 273 show
14 Results NF-κB phosphorylation It is well established that TNF-α is one of the major stimuli toward phosphory 26 971 773 show
15 Results SMI-71 expression Previously, we reported that SMI-71 (an endothelial barrier antigen) immunoreactiv 26 934 492 show
16 Results Neutrophil infiltration Recent studies have reported that neutrophils infiltrate the brain under cer 26 1212 509 show
17 Results MIP-2 expression MIP-2 is a powerful chemokine that contributes to recruitment of neutrophils [27]. 26 1575 946 show
18 Discussion The major findings in the present study are that TNF-α signaling showed cellular specific responses 26 5553 3149 show
19 Caption-Figure 1 Expression of TNF-α and TNF receptor in the PC 12 hr-post SE. (A) TNF-α immunoreactivity in Ox-42-po 26 270 228 show
20 Caption-Figure 2 Effect of sTNFp55R infusion on SE-induced serum-protein extravasation and neuronal damage. (A-D) Ser 26 573 250 show
21 Caption-Figure 3 Effect of sTNFp55R infusion on NF-κB phosphorylation in astrocytes 12 hr after SE. In 12 hr-post SE 26 466 501 show
22 Caption-Figure 4 Quantitative analyses of the effect of sTNFp55R infusion on NF-κB phosphorylation and SMI-71 express 26 493 251 show
23 Caption-Figure 5 Effect of sTNFp55R infusion on p65-Thr435 phosphorylation in endothelial cells following SE. (A-B) I 26 768 538 show
24 Caption-Figure 6 Effect of sTNFp55R infusion on neutrophil infiltration and MIP-2 expression following SE. (A) Neutro 26 1036 590 show