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PMC:3291650 JSONTXT

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0 TIAB A20 (Tnfaip3) Deficiency in Myeloid Cells Protects against Influenza A Virus Infection The innate im 33 1527 659 show
1 Introduction Viruses are a class of highly diverse pathogens which depend on the host cell for their replication. 26 6561 3757 show
2 Results A20 inhibits RIG-I-induced NF-κB and IRF3 activation RIG-I signaling induces the activation of NF-κB 26 4394 3207 show
3 Results A20 negatively regulates IAV-induced gene expression in BMDM To investigate the role of A20 in the I 26 1534 949 show
4 Results A20 deficiency in myeloid cells protects against IAV lung infection To determine the role of A20 exp 26 6116 2964 show
5 Discussion In the present study we have investigated the contribution of A20 expression in myeloid cells in the 26 6699 3259 show
6 Materials and Methods Ethics statement All experiments on mice were conducted according to the national (Belgian Law 14/08 26 528 194 show
7 Materials and Methods Mice A20fl/fl mice were generated as previously described [56]. A20fl/fl mice were crossed with LysM 26 734 288 show
8 Materials and Methods Viral infection and determination of viral titers Mouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) 26 967 379 show
9 Materials and Methods Determination of HAI (hemagglutination inhibition) titers To determine the HAI titers in infected mi 26 1006 414 show
10 Materials and Methods In vivo intracellular GrB and IFNγ staining of activated CD8+ T cells Granzyme B (GrB) and IFNγ expr 26 696 461 show
11 Materials and Methods Cells and transfection HEK293T and MDCK cells were grown in DMEM (Gibco) supplemented with 10% FCS, 26 1428 628 show
12 Materials and Methods Western blotting For total lysates, cells were lysed at 4°C for 15 min in lysis buffer (200 mM NaCl, 26 1326 632 show
13 Materials and Methods Flow cytometry Lungs were dissected and incubated with collagenase type IV (1 mg/ml; Sigma) and DNAs 26 896 442 show
14 Materials and Methods Cytokine quantification For TNF ELISA, 96-well plates were coated with TNF coating (TN3-19, eBioscie 26 600 397 show
15 Materials and Methods RNA isolation, cDNA synthesis and qPCR Total RNA was extracted using Aurum Total RNA mini kit (BioRa 26 1035 468 show
16 Materials and Methods Statistics Results are expressed as the mean ± SEM. Statistical significance between groups was asse 26 444 161 show
17 Caption-Figure 1 A20 inhibits NF-κB and IRF3 activation in response to RIG-I stimulation. (A) HEK293T cells were tran 26 1325 983 show
18 Caption-Figure 2 A20 negatively regulates IAV-induced gene expression in BMDM and alveolar macrophages. (A–B) BMDM is 26 834 516 show
19 Caption-Figure 3 A20 deficiency in myeloid cells protects against IAV lung infection. A20myel-WT and A20myel-KO mice 26 822 437 show
20 Caption-Figure 4 A20 deficiency in myeloid cells protects against lethal IAV infection. A20myel-WT (n = 11) and A20my 26 743 426 show