Animals
Srf (flex1neo/flex1neo) and Camk2α-iCre mice were bred to obtain Srf mutants (Srf–/–; Camk2a-iCre) or control littermates (Srf –/–, Srf +/– or Srf +/–; Camk2α-iCre [35], [36]). Recombination induced by Cre recombinase expression via the Camk2α promoter starts just before birth and results in strong SRF down-regulation at time-points used to culture primary neurons [35], [36]. EphA7 mice were kindly provided by U. Drescher (King's College, London, UK; see [15]). Animal experiments and housing were approved by the local ethics committee (Einrichtung für Tierschutz, Tierärztlichen Dienst und Labortierkunde, Calwer Straße 7/4, 72076 Tübingen, Tübingen University; permit number: §4 Anzeige 15/10/2009).