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div. section text # proj. length # Ann.
0 TIAB Ephrin-A5 Suppresses Neurotrophin Evoked Neuronal Motility, ERK Activation and Gene Expression Durin 1 1978 0 show
1 Introduction During brain development, axons encounter attractive and repulsive guidance cues, whose interplay in 1 3911 0 show
2 Materials and Methods Animals Srf (flex1neo/flex1neo) and Camk2α-iCre mice were bred to obtain Srf mutants (Srf–/–; Camk2a 1 711 0 show
3 Materials and Methods Neuronal cell culture P1 hippocampal or E17.5 cortical cultures were incubated in NMEM/B27 medium as 1 1007 0 show
4 Materials and Methods Biochemistry Cortical cultures were stimulated with 1 µg/ml clustered (see above) ephrin-A5-Fc and/o 1 1116 0 show
5 Materials and Methods Quantitative real-time PCR (qRT-PCR) Cultures were stimulated with 2.5 ng/ml BDNF and/or 1 µg/ml pre 1 529 0 show
6 Materials and Methods Immunocytochemistry Cells were fixed for 15 minutes in 4% PFA/5% Sucrose/PBS, permeabilized for 5 mi 1 947 0 show
7 Materials and Methods Image acquisition and statistics Pictures were acquired on a Zeiss Axiovert 200M or Zeiss LSM confoc 1 1349 0 show
8 Results Ephrin-A5 stimulation counteracts BDNF-induced neuronal motility in vitro in an SRF-dependent manner 1 2752 0 show
9 Results Ephrin-As counteract BDNF signaling via suppression of ERK kinase activity Next, we investigated the 1 2658 0 show
10 Results Eph forward signaling induces ERK activation within the neurites while impairs BDNF induced p-ERK ac 1 1780 0 show
11 Results Eph forward signaling modulates BDNF-evoked IEG expression Nuclear P-ERK enhances gene transcription 1 2612 0 show
12 Results ERK signaling is involved in decreasing BDNF-induced gene activity via Eph receptors Data provided s 1 4227 0 show
13 Results EphA7 modulates growth cone morphology yet is dispensable for ephrin-neurotrophin communication In o 1 2454 0 show
14 Discussion In this study, we explored consequences of primary neurons stimulated simultaneously with two guidan 1 6031 0 show
15 Caption-Figure 1 Ephrin-A5 treatment blocks BDNF-induced growth cone motility in an SRF-dependent manner. Wild-type a 1 1785 0 show
16 Caption-Figure 2 Ephrin-A5 suppresses ERK activation and inhibits BDNF-evoked ERK phosphorylation. (A) Cortical neuro 1 1246 0 show
17 Caption-Figure 3 Eph forward signaling prevents nuclear ERK translocation. Wild-type hippocampal neurons were treated 1 1417 0 show
18 Caption-Figure 4 The BDNF-evoked neuronal IEG response is counteracted by ephrin-A5 co-application. Wild-type (black 1 1228 0 show
19 Caption-Figure 5 Constitutively-active MEK1 blocks ephrin-A5 mediated growth cone collapse. Wild-type neurons were el 1 1391 0 show
20 Caption-Figure 6 Ephrin-A5 suppresses BDNF-induced IEG responses via MAP kinase signaling. Wild-type cortical neurons 1 703 0 show
21 Caption-Figure 7 EphA7 modulates growth cone morphology yet is dispensable for ephrin-neurotrophin communication. Hip 1 1676 0 show