Comparative transcriptomic analysis of EA 2018 and ATCC 824
To further explore the molecular mechanism of enhanced butanol production in the EA 2018 strain, DNA microarray of C. acetobutylicum were manufactured and used for a comparative analysis between EA 2018 and ATCC 824. The complete set of DNA array data was available in Additional file 8. Microarray analysis showed that a total of 2215 genes were differentially regulated at transcriptional level in at least 1 cultivation time point. Among them, differentially regulated genes related to some important metabolic pathways were listed in Table 4. And some putative promoter variation genes such as adhEII were found with differential expression level in the EA 2018 strain (Table 5). The detailed comparative analysis of transcriptomic data, along with genomic data (i.e. variation gene sequence) and biochemical phenotypes will be provided below from three key aspects related to enhanced butanol production (i.e. solvent formation, xylose utilization, and spore formation).
Table 4  List of differentially regulated genes in key functional groups.
Table 5  DNA and transcriptional variations of putative promoter variations between EA 2018 and ATCC 824

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