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PMC:2889865 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB Differential cytokine regulation by NF-κB and AP-1 in Jurkat T-cells Background Activator protein (A 28 2457 1336 show
1 Background Cytokines and chemokines are important in immune cell recruitment and in regulation of inflammatory 21 2846 1713 show
2 Results Regulation of AP-1 and NF-κB activation The transcription factors NF-κB and AP-1 play key roles in t 21 1988 810 show
3 Results Induction of inflammatory responses The ability of PMA and HK E. coli to induce an inflammatory resp 21 1291 745 show
4 Results Cooperative induction of cytokines by AP-1 and NF-κB To further characterize the involvement of NF-κ 21 2019 1343 show
5 Results NF-κB inhibition due to PKC-dependent Bcl10 degradation Western blot analysis revealed an up-regulat 21 787 476 show
6 Discussion NF-κB and AP-1 are critical regulators of inflammatory responses, proliferation and differentiation 21 8815 4965 show
7 Conclusion In the present study, IL-6 release was found to be associated with NF-κB activity while CXCL8 releas 21 915 582 show
8 Materials and methods Chemicals The following chemicals were used in the present study: PMA (Phorbol 12-myristate 13-aceta 21 470 218 show
9 Materials and methods Heat killed (HK) Escherichia coli E. coli MG1655 were grown on Luria-Bertani (LB) agar and incubated 21 597 275 show
10 Materials and methods Cell culturing, transfection and stimulation Jurkat T-cells (wild type and TCR deficient- TCR-/-) we 21 1680 732 show
11 Materials and methods Multiplex cytokine assay Quantification of the levels of cytokines IL-2, IL-6, IL-10 and TNF and the 21 528 239 show
12 Materials and methods Enzyme-linked immunosorbent assay (ELISA) ELISA was performed on supernatants from challenged Jurkat 21 713 354 show
13 Materials and methods Western blot analysis Following stimulation, Jurkat T-cells were centrifuged at 1000 × g for 8 min a 21 800 335 show
14 Materials and methods RNA extraction Jurkat T-cells were treated with NF-κB, JNK and PKC inhibitors for 2 h in 6-well plat 21 856 363 show
15 Materials and methods Reverse transcription quantitative PCR (RT-qPCR) RT-qPCR was used to determine gene expression level 21 903 474 show
16 Materials and methods Statistical analysis Statistical significant differences were determined using two-tailed Student's 21 145 59 show
17 Caption-Figure 1 AP-1 activation following long-term exposure of Jurkat T-cells to PMA. Jurkat T-cells were transfect 21 624 280 show
18 Caption-Figure 2 NF-κB down-regulation by PMA and up-regulation by heat killed E. coli MG1655 following long-term sti 21 651 273 show
19 Caption-Figure 3 CXCL8, IL-6 and TNF expression following long-term stimulation with PMA. Jurkat T-cells were either 21 495 295 show
20 Caption-Figure 4 Inhibition of NF-κB activity by NAI. Jurkat T-cells were transfected with luciferase reporter plasmi 21 516 258 show
21 Caption-Figure 5 Involvement of NF-κB in cytokine regulation. Cytokine/chemokine levels were determined using ELISA f 21 757 286 show
22 Caption-Figure 6 Involvement of PKC and JNK in cytokine expression. Jurkat T-cells were incubated with PKC- and JNK i 21 496 298 show
23 Caption-Figure 7 NF-κB inhibition by PMA correlated to PKC-dependent Bcl10 degradation. Levels of intracellular prote 21 541 295 show
24 Caption-Table 1 Jurkat T-cells were stimulated with 162 nM PMA and 5 × 107 CFU/ml HK E. coli for 24 h. 21 86 41 show
25 Caption-Table 2 Jurkat T-cells were treated with 10 nM NAI, 10 μM JNK-I or10 nM PKC-I for 2 h followed by induction 21 125 65 show