The temporal changes of TLR4 mRNA in brain tissue after ICH. TLR4 mRNA levels were detected by Rt-PCR. The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA. As shown in Figure 1, the low level of TLR4 mRNA was observed in normal rat brain tissue. In the saline control group, the TLR4 mRNA slightly increased but was no significantly different from normal group. However, the TLR4 mRNA after ICH significantly increased starting from 6 hours after ICH (P <.05), peaked on 3 days after ICH (P < .05), and then decreased on 7 days after ICH, but still maintained at a higher level compared with saline control (P < .05). #: compared with saline control, P < .05; *: compared with 6 hours after ICH, P < .05.