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div. section text # proj. length # Ann.
0 TIAB Activation of TLR4-Mediated NFκB Signaling in Hemorrhagic Brain in Rats Inflammation and immunity pl 33 1369 788 show
1 1. Introduction Intracerebral hemorrhage (ICH) represents 10%–15% of all strokes and occurs with increasing frequenc 26 2131 973 show
2 2. Methods 2.1. Animals Ninety Wister rats (male, body weight: 250 g to 300 g) were provided by Experimental An 26 1130 467 show
3 2. Methods 2.2. Induction of Intracerebral Hemorrhage Since most intracerebral hemorrhages occur in the basal g 26 1140 450 show
4 2. Methods 2.3. Detection of TLR4 mRNA by the Method of RT-PCR Briefly, total RNA samples were isolated from pe 26 1516 825 show
5 2. Methods 2.4. Immunohistochemistry (IHC) The IHC staining for TLR4 was performed as described previously [8]. 26 415 203 show
6 2. Methods 2.5. Western Blots Cellular proteins were prepared from ICH cerebral hemispheres, electrophoresed wi 26 704 284 show
7 2. Methods 2.6. Electrophoretic Mobility Shift Assay (EMSA) Nuclear proteins were isolated from brain tissue. B 26 892 389 show
8 2. Methods 2.7. Statistical Analysis All data were presented as mean ± SEM. The measurements were analyzed by o 26 231 94 show
9 3. Results 3.1. TLR4 mRNA Increased in Brain Tissue after ICH TLR4 mRNA levels were detected by Rt-PCR. The low 26 552 375 show
10 3. Results 3.2. Expression of TLR4 Protein Increased in Brain Tissue after ICH To investigate the increased TLR 26 673 488 show
11 3. Results 3.3. Distribution of TLR4 in Brain Subjected to ICH To explore the distribution of TLR4 protein, the 26 430 252 show
12 3. Results 3.4. Activation of TLR4-Mediated NF-Kappa-B Signaling Activation of TLR4 induces the phosphorylation 26 766 444 show
13 4. Discussion Intra-cerebral hemorrhage (ICH) represents 10–15% of all strokes, and occurs with increasing frequen 26 6248 3007 show