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div. section text # proj. length # Ann.
0 TIAB GATA3-Driven Th2 Responses Inhibit TGF-β1–Induced FOXP3 Expression and the Formation of Regulatory T 28 1441 811 show
1 Introduction Effective immune responses are characterized by T cell activation, which directs adaptive and innate 21 3424 1815 show
2 Results FOXP3 Induction in T Cell Subsets It is assumed that FOXP3 expression can be induced in nonregulator 21 3265 2445 show
3 Results FOXP3 and GATA3 Kinetics in Differentiating Cells The limited capacity of differentiated effector ce 21 1799 1130 show
4 Results IL-4 Inhibits TGF-β-Mediated iTreg Commitment IL-4 induces differentiation of naive T cells, upon an 21 2702 1923 show
5 Results The Role of TGF-β in T Cell Differentiation Although TGF-β–reduced CD25, IL-4 expression, and CD25 e 21 2070 1454 show
6 Results GATA3 Is a Negative Regulator of FOXP3 Expression FOXP3 expression decreased once GATA3 expression i 21 2335 1780 show
7 Results GATA3 Represses the FOXP3 Promoter To investigate the molecular mechanism of GATA3-mediated repressi 21 2469 1664 show
8 Discussion The current study reveals that FOXP3 induction, an important step in iTreg commitment, is inhibited 21 7521 4728 show
9 Materials and Methods Mice. Normal B6 mice were purchased from the Jackson Laboratories (Bar Harbor, Maine). Transgenic DO 21 609 300 show
10 Materials and Methods Isolation of CD4+ T cells. CD4+ T cells were isolated from blood of healthy human volunteers using t 21 415 253 show
11 Materials and Methods Quantitative real-time PCR. The PCR primers and probes were designed based on the sequences reported 21 1582 458 show
12 Materials and Methods Inducible murine Treg culture. Naive CD4+ T cells (CD4+, CD62L+, and CD25–) were isolated from poole 21 505 296 show
13 Materials and Methods In vitro T cell differentiation. CD4+ CD45RA+ magnetically-sorted (CD45RO depletion, MACS, according 21 569 445 show
14 Materials and Methods Cloning of the FOXP3 promoter and construction of mutant constructs. The FOXP3 promoter was cloned i 21 472 238 show
15 Materials and Methods Transfections and reporter gene assays. T cells were rested in serum-free AIM-V medium (Life Technol 21 636 301 show
16 Materials and Methods RNA isolation and cDNA synthesis. RNA was isolated using the RNeasy Mini Kit (Qiagen) according to t 21 307 108 show
17 Materials and Methods Recombinant TAT proteins. The cDNAs encoding GATA3 protein or the truncated GATA3 (lacking the two z 21 463 256 show
18 Materials and Methods TAT-GATA3 transduction. CD4+CD45RA+ cells were cultured in AIMV medium and transduced with 20 nM, 10 21 405 301 show
19 Materials and Methods Intracellular cytokine staining. T cells were stimulated with 2 × 10−7 M PMA and 1 μg/ml of ionomyci 21 422 182 show
20 Materials and Methods Flow cytometry. For analysis of FOXP3 expression at the single-cell level, cells were first stained 21 1665 865 show
21 Materials and Methods Western blotting. For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and lo 21 906 467 show
22 Materials and Methods Administration of cytokines and antibodies in vivo. Age- and gender-matched normal B6 mice received 21 529 338 show
23 Materials and Methods ChIP. ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) w 21 1013 402 show
24 Materials and Methods Pull-down assay. CD4+ T cells were stimulated with PMA and ionomycin for 2 h at 37°C. The cells were 21 1002 467 show
25 Caption-Figure 1 Th2 Cells Cannot Induce FOXP3 Expression (A) Human T cells were activated with plate-bound anti-CD3/ 21 567 359 show
26 Caption-Figure 2 Th2 Cells Do Not Express FOXP3 (A) Intracellular FACS analysis of FOXP3 expression in Th1, Th2, or i 21 473 260 show
27 Caption-Figure 3 Th2- or IL-4–Producing Cells Lack FOXP3 (A) FACS analysis of intracellular FOXP3 and IL-4 expression 21 777 600 show
28 Caption-Figure 4 FOXP3 Induction During the Differentiation Process (A) Human CD4+CD45RA+ T cells were activated with 21 569 413 show
29 Caption-Figure 5 Effect of IL-4 on FOXP3 Induction (A) A statistical analysis was performed with six donors on day 5 21 1045 761 show
30 Caption-Figure 6 IL-4 Inhibits TGF-β–Mediated iTreg Commitment CFSE-labeled CD4+CD45RA+ cells were activated with pla 21 612 446 show
31 Caption-Figure 7 GATA3 Acts as a Negative Regulator of FOXP3 Expression (A) Human naive CD4+CDRA+ T cells were transd 21 865 589 show
32 Caption-Figure 8 GATA3 Represses the Human FOXP3 Promoter (A) Jurkat, Th2 cells, and human primary CD4 cells were tra 21 1675 870 show