Wt1 and Cited1 (red) staining revealed an increase of approximately 30% in the thickness of the capping mesenchyme surrounding the mutant ureteric buds (Figure 3, Table 2). Nevertheless, the mutant metanephric mesenchyme underwent relatively normal nephrogenesis. Cdh1-staining nephrons (blue) were identified connecting to the ureteric bud tips [16] and extending into the medulla of the kidney, as normal. Intermediate structures of nephrogenesis, including renal vesicles, comma-shaped bodies, and S-shaped bodies appeared normal.