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0 TIAB Pygo1andPygo2roles in Wnt signaling in mammalian kidney development Background The pygopus gene of D 5 2434 1372 show
1 Background Wnt signaling is of critical importance in several stages of kidney development. Mutual inductive in 5 3006 1836 show
2 Results Pygo1 and Pygo2 targeted mutations We targeted both the Pygo1 and Pygo2 genes by inserting LoxP sequ 5 651 414 show
3 Results Pygo1 and Pygo2 mutant phenotypes Pygo1 homozygous null mice were viable and fertile with no develop 5 1205 695 show
4 Results Expression of Pygo1 and Pygo2 in the developing kidney In situ hybridization has been used previousl 5 699 447 show
5 Results Confocal analysis of Pygo1/Pygo2 mutant kidneys Histological examination of Pygo2 null and Pygo1/Pyg 5 1885 1560 show
6 Results In situ hybridization We examined expression of three Wnt genes in Pygo mutants. Wnt7b is expressed 5 543 378 show
7 Results Reduced canonical Wnt signaling in Pygo1/Pygo2 mutant mice The BAT-gal transgene reporter of canonic 5 5775 5625 show
8 Results Microarray analysis of Pygo1/Pygo2 null kidneys To further examine possible disturbance of gene expr 5 3284 2271 show
9 Discussion In Drosophila, the Pygopus gene is a key mediator of canonical Wnt signaling. In one study, 12 disti 5 6474 3784 show
10 Conclusion In conclusion, the mammalian Pygo2 gene is required for normal branching morphogenesis of the ureter 5 1615 946 show
11 Methods Targeting of Pygo1 and Pygo2 Pygo1 and Pygo2 genes were each targeted by flanking the critical codin 5 2022 1119 show
12 Methods Confocal microscopy Kidneys were dissected, fixed in paraformaldehyde, treated with methanol, and wa 5 1317 853 show
13 Methods In situ hybridization Whole-mount in situ hybridization was performed as previously described [35]. 5 213 140 show
14 Methods Pygo1 and Pygo2 antibody production To generate anti-human Pygo2 (anti-hPygo2) and anti-mouse Pygo1 5 1121 753 show
15 Methods BAT-gal transgene reporter assay of canonical Wnt signaling X-gal staining of both embryos and devel 5 662 511 show
16 Methods Microarray analysis Total RNA was isolated from E18.5 kidneys dissected from normal and Pygo1/Pygo2 5 1034 603 show
17 Methods Quantitative PCR validation of microarray results Total RNA from E18.5 Pygo1/Pygo2 null and control 5 1427 698 show
18 Caption-Figure 1 Pygo1/Pygo2 mutant embryos. (A) E18.5 Pygo1-/-/Pygo2+/- embryos, with only one wild-type Pygo2 allel 5 362 286 show
19 Caption-Figure 2 Pygo1 and Pygo2 expression in the E18.5 developing kidney. Immunofluorescence was used to determine 5 565 361 show
20 Caption-Figure 3 Confocal analysis of Pygo1/Pygo2 mutant E18.5 kidneys. (B, D) Pygo1-/-/Pygo2-/- mutant E18.5 kidneys 5 803 558 show
21 Caption-Figure 4 Expression of Wnt7b, Wnt9b, and Wnt11 in E18.5 Pygo1/Pygo2 compound null kidneys. Whole-mount in sit 5 472 381 show
22 Caption-Figure 5 Reduced canonical Wnt signaling in Pygo2 and Pygo1/Pygo2 mutant embryos. E10.5 embryos, all with the 5 1109 818 show
23 Caption-Figure 6 Pygo2 is required for BAT-gal reporter expression in ureteric bud-derived structures of the developi 5 2506 2133 show
24 Caption-Figure 7 Quantitative analysis of BAT-gal reporter expression in Pygo1/Pygo2 E18.5 kidney extracts. Transgene 5 559 410 show
25 Caption-Figure 8 Gene expression changes of common Wnt signaling targets in the E18.5 Pygo1/Pygo2 null kidney. Microa 5 555 335 show
26 Caption-Table 1 Phenotypes of Pygo2 wild-type, heterozyogous, and null E18.5 embryos on a Pygo1 null background. 5 96 64 show
27 Caption-Table 2 Confocal analysis of Pygo2 wild-type, heterozygous, and null E18.5 kidneys on the Pygo1 null backgro 5 104 65 show
28 Caption-Table 3 Genes differentially expressed (p < 0.05, two-fold change or greater) in the Pygo1/Pygo2 null E18.5 5 139 93 show
29 Caption-Table 4 Validation of gene expression changes in the E18.5 Pygo1/Pygo2 null kidneys normalized to E18.5 wild 5 114 76 show