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PMC:1853120 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB A Mammal-SpecificDoublesexHomolog Associates with Male Sex Chromatin and Is Required for Male Meiosi 6 1858 1210 show
1 Introduction Sexual differentiation generates anatomical, physiological, and behavioral dimorphisms that are esse 6 6424 4285 show
2 Results Expression of DMRT7 Proteins in Testis Our previous mRNA expression analysis suggested a possible me 6 3395 2308 show
3 Results Targeted Deletion of Dmrt7 To establish the functional requirement for Dmrt7, we generated Dmrt7−/− 6 1637 1149 show
4 Results Dmrt7 Is Required for Male but Not Female Gametogenesis Breeding of Dmrt7 heterozygotes produced hom 6 2932 1857 show
5 Results Pachytene Arrest of Dmrt7 Mutant Germ Cells To better define the spermatogenic stage at which Dmrt7− 6 1530 1008 show
6 Results Normal Meiotic Prophase in Dmrt7 Mutant Germ Cells Defects in chromosome pairing, synapsis, or recom 6 1299 811 show
7 Results Abnormal Cellular Organization and the Role of Sertoli Cells Sertoli cells interact with germ cells 6 2356 1595 show
8 Results XY Bodies Form Normally in Dmrt7 Mutant Cells The data presented so far indicate that Dmrt7 mutant g 6 2514 1749 show
9 Results Abnormal Sex Chromatin in Dmrt7 Mutant Germ Cells Although the XY body can form during pachynema in 6 4649 3104 show
10 Discussion In this study, we find that the DM domain protein DMRT7 is required for male germ cells to complete 6 7663 4968 show
11 Materials and Methods Generation of Dmrt7 mice. A mouse Dmrt7 cDNA fragment containing sequences from exon 8 was used to s 6 1981 1351 show
12 Materials and Methods Genotyping and RT-PCR. For genotyping, tail-clip DNA was amplified for 35 cycles. Chromosomal sex wa 6 612 450 show
13 Materials and Methods Primers. DM5S3: 5′-AGAGTGGATTGAATCGGATAGCTC-3′ DM5S4: 5′-AGGATCTTAGTGTCGAATGAATAC-3′ DM5S5: 5′-CCCTT 6 584 378 show
14 Materials and Methods Histological analysis and TUNEL assay. Dissected testes were fixed in Bouin's fixative or phosphate- 6 634 349 show
15 Materials and Methods Tissue immunofluorescent staining. Slides with paraffin sections were washed in PBT (0.1% Tween 20 i 6 397 243 show
16 Materials and Methods Antibodies. Rabbit polyclonal antibodies to DMRT7 were raised against a purified fusion protein cont 6 1853 1396 show
17 Materials and Methods Meiotic chromosome spread preparations and FISH. Meiotic chromosome spread preparations were made fr 6 755 423 show
18 Caption-Figure 1 Expression of Dmrt7 mRNA and Protein (A) RT-PCR analysis of Dmrt7 mRNA from ten organs of adult mous 6 808 658 show
19 Caption-Figure 2 Reduced Testis Size and Germ Cell Apoptosis in Mice with Targeted Deletion in Dmrt7 (A) Testes from 6 1006 818 show
20 Caption-Figure 3 Prophase I Arrest of Dmrt7 Mutant Germ Cells Testis sections from 6-wk-old wild-type (+/+) and Dmrt7 6 482 370 show
21 Caption-Figure 4 Profile of Meiotic Arrest in Dmrt7 Mutant Testis Graph of distribution of meiotic stages of germ cel 6 309 219 show
22 Caption-Figure 5 Normal Synapsis and Recombination in Dmrt7 Mutant Germ Cells (A) Testicular cells from Dmrt7 heteroz 6 658 479 show
23 Caption-Figure 6 Abnormal Sertoli Cell Organization in Dmrt7 Mutant Testes (A) Testes from P14 wild-type and Dmrt7 mu 6 1064 816 show
24 Caption-Figure 7 XY Body Forms Normally during Pachynema in Dmrt7 Mutant Mice (A) Testicular cells spread and stained 6 494 412 show
25 Caption-Figure 8 Chromatin Abnormalities in Dmrt7 Mutant Diplotene Germ Cells (A and B) MSCI occurs normally in mid-p 6 1273 1022 show
26 Caption-Figure 9 Abnormal Sex Chromatin in Cells Staged by Chromosome Pairing Status (A) Spread of wild-type germ cel 6 441 293 show