For histology and section in situ hybridization, samples were fixed in 4% paraformaldehyde in PBS at 4 °C overnight. Newborn and older animals were then decalcified in Tris buffer containing 10% EDTA and 7.5% polyvinylpyrrolidone (pH 7.5) at 4 °C for 3 wks. Samples were then dehydrated through a graded ethanol series, cleared in xylene, and embedded in paraffin. 8μm sections were collected and stained with hematoxylin and eosin (H & E) or toluidine blue following standard procedure.