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PMC:1630711 JSONTXT

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div. section text # proj. length # Ann.
0 TIAB CAF-1 Is Essential for Heterochromatin Organization in Pluripotent Embryonic Cells During mammalian 5 1940 1142 show
1 Introduction During mouse pre-implantation development, the genome undergoes a series of major epigenetic changes 5 2582 1632 show
2 Results We used gene targeting in ES cells to delete exon 3 in the Chaf1a gene, which encodes p150CAF-1 (Fig 5 12492 7606 show
3 Discussion Here, we provide evidence that p150CAF-1 has a crucial function during mouse early embryogenesis and 5 6503 3938 show
4 Materials and Methods Generation of Chaf1a mutant mice and embryos. Using PCR, we amplified two genomic fragments (about 3 5 1137 741 show
5 Materials and Methods P150CAF-1 depletion by RNAi. The RNAi plasmid vector that we used in this study contains the mouse H 5 1450 938 show
6 Materials and Methods Cell lines. AT-1 ES cells [42] (a gift of M. Vernet) were used for gene targeting. LTM7 ES cells wer 5 339 263 show
7 Materials and Methods Immunofluorescence. Cells were fixed for 20 min in PBS with 4% paraformaldehyde, and immunodetection 5 830 488 show
8 Materials and Methods Western blot analysis. Cells were lysed in Laemli buffer and run onto a 4%–12% SDS PAGE gradient gel 5 277 262 show
9 Materials and Methods DNA FISH. Probes were described previously [20]. Biotin-16-dUTP or Digoxigenin-11-dUTP (Roche, Basel 5 404 245 show
10 Materials and Methods Nuclei preparation, nuclease digestion, and biochemical analysis of chromatin. ES cells were incubat 5 1310 989 show
11 Materials and Methods ChIP. We prepared native chromatin fragments of two to six nucleosomes in length as described [28]. 5 952 583 show